Immobilizing Redox Enzyme on Amino Functional Group-Integrated Tailor-Made Polyester Textile: High Loading, Stability, and Application in a Bio-Fenton System

This study reports the first approach of immobilizing a redox (glucose oxidase-GOx) enzyme on amino functional group-integrated tailor-made textile (polyester nonwoven fabric-PF) support matrix. To achieve that, polyethylenimine if not chitosan was chemically grafted plasma (with O2/N2 gas)-activated PF before GOx through physical adsorption. Diverse qualitative and quantitative characterization methods were used to validate successful activation immobilization PF. Results showed that integration groups offers great deal favorable conditions during covalent or ionic interaction between counter as reflected in high loading (55.46%) good operational (78.37%) thermal stability (∼60 °C) with excellent recyclability (60% activity/15-cycles) poor leaching (22%) immobilized GOx. Enzymatic reaction kinetics free revealed existence relative mass transfer diffusion limitation apprehended apparent Michaelis constant (Km) maximum velocity (Vmax). The resultant GOx’s studied for time removal pollutants (10 mg L–1 crystal violet) from water heterogeneous bio-Fenton system. 88.69% pollutant at 1.19 × 10–2 min–1 following pseudo-first-order kinetic model supported by R2 values beyond 97. These results are importance they provide fundamental evidence proof concepts regarding biocatalysts textiles their potential application robust catalytic system environmental green chemistry applications.